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Dernière mise à jour : Mai 2018

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Lipoprotein and polysaccharide-protein structures (ELIPS)

ELIPS

              Context and Objectives - Research lines - Methods and Equipment

                          Recent work - Patents ans Thesis - Publications

     

Team leader: Didier MARION

Equipe ELIPS

Permanent team members:

Bénédicte Bakan, Michèle Dalgalarrondo, Khalil Emorjani, Nathalie Geneix, André Lelion,  Didier Marion, Anne-Lyse Panheleux

Temporary team members:

Coralie Prin

Administrative staff:

Céline Migot, Isabelle Mimbre

 

Context and Objectives

Processing and nutritional quality of plant products depends in part on the assembly of the various components within the plant organs harvested. In this context we focus specifically on the one hand to the structure and assembly of the cuticles of fruits (including caryopses of cereals) and secondly, to the formation of amyloid-protein matrix grains cereals. Our goal is to characterize the major cellular, molecular and physicochemical mechanisms accompanying the formation of these supramolecular plants to identify new markers closely combining plant breeding and technology food or non-food processing.

  

Research lines

  • Formation and structure of cuticles: relationship with the processing and quality of plant products
  • Formation et structure of the amyloid-protein matrix grain: relationship with the processing of cereals

   

Methods et Equipment

  • Analysis and purification of proteins and lipids: 1D and 2D electrophoresis, HPLC analytical and semi-preparative reversed phase and normal phase, ion exchange chromatography and size exclusion, affinity chromatography, gas chromatography coupled with mass spectrometry, HPLC coupled to mass spectrometry.
  • Cloning, PCR, production and purification of recombinant proteins
  • Immunochemical methods (ELISA, immunoblotting) and immunocytochemistry (coupled with confocal microscopy, transmission electron microscopy)
  • Structural analysis: circular dichroism, fluorescence spectroscopy and infrared

    

Recent work

  • Highlighting the role of GDSL-lipase/acylhydrolase in the formation of cutin of tomato fruit
  • Puroindolines and hardness of wheat: New Perspectives
  • Towards sustainable processes in malting

       

Keywords

Protein, fat, cuticle, cereals, fruits, development, aggregation, polymerization interactions, biosynthesis